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How To Make A Blood Smear. Use clean high-quality microscope slides. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. In this video I quickly go over how to make a peripheral blood smear. Can occur with anemic patients Increase the angle of the slide you are using to smear with.
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Select the finger to puncture usually the middle or ring finger. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells. Collect blood in an EDTA tube and make the smears when back at the clinic. A well made blood smear consists of several areas. How to Make a Peripheral Blood Smear. Clean the area to be punctured with 70 alcohol.
The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide.
3 Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. Select the finger to puncture usually the middle or ring finger. Place either the long or the short edge of the pusher slide just in front of the blood drop holding it at an approximately 30-degree angle from the patient slide. I hope you can see the smear well enough in the video if not please leave a comment and. A well made blood smear consists of several areas. Optimise spreading speed for length and a good feathered edge.
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Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide. Blood can also be taken from an EDTA sample. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. The feathered edge the monolayer and the body and base of the smear.
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Spreader slide pushed across the slide in a jerky. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. Select the finger to puncture usually the middle or ring finger. If the blood has been refrigerated allow it to return. All parts of the smear should be examined however the monolayer is the area where the cells are examined in close detail and differential cell.
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Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide. Place a small drop of blood or one side about 1-2 cm from one end. Optimise spreading speed for length and a good feathered edge. Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. A phlebotomist a person specifically trained to draw blood first cleans and sterilizes the injection site with an antiseptic.
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Now push the spreader across the slide. The main part of the blood film. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. The portion of blood film near the drop of blood. The feathered edge the monolayer and the body and base of the smear.
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Without delay place a spreader at an angle of 45 from the slide and move it back to make contact with the drop. Preparation of Blood Film. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. How to perform a blood film in 3 easy stepsLearn more and visit httpswwwidexxcouk.
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Can occur with anemic patients Increase the angle of the slide you are using to smear with. Without delay place a spreader at an angle of 45 from the slide and move it back to make contact with the drop. The scratches allow for improved adherence of the blood film to the slide without affecting the smear morphology. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. Clean slides with 70 to 90 alcohol and allow to dry.
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Push carefully along the length of the first slide to produce a thin smear of blood. Can occur with anemic patients Increase the angle of the slide you are using to smear with. Take one drop of venous or capillary blood. Take another clean slide and holding at an angle of about 45 deg touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action. The tapering end of the blood film.
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Spreader slide pushed across the slide in a jerky. Prick the ring finger of left hand with the help of prickling needle under all aseptic conditions discard the first two drOps of blood from skin puncture site. The feathered edge the monolayer and the body and base of the smear. The tapering end of the blood film. Push carefully along the length of the first slide to produce a thin smear of blood.
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Can occur with anemic patients Increase the angle of the slide you are using to smear with. The tapering end of the blood film. The feathered edge the monolayer and the body and base of the smear. To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. Take one drop of venous or capillary blood.
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Making a quality blood smear Although there are several techniques described for making blood smears most people use the wedge or push technique. Components of a blood smear. Can occur with anemic patients Increase the angle of the slide you are using to smear with. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells. Aim for a blood droplet size of 4mm diameter.
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Prick the ring finger of left hand with the help of prickling needle under all aseptic conditions discard the first two drOps of blood from skin puncture site. If the blood has been refrigerated allow it to return. A quick instructional video demonstrating. Place either the long or the short edge of the pusher slide just in front of the blood drop holding it at an approximately 30-degree angle from the patient slide. The tapering end of the blood film.
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To make a short smear hold the spreader at a steeper angle and to make a longer smear hold it closer to the drop. Blood can also be taken from an EDTA sample. The main part of the blood film. A quick instructional video demonstrating. If the blood has been refrigerated allow it to return.
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The slide should be clean. Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. How to Make a Peripheral Blood Smear. The slide should be clean. The feathered edge the monolayer and the body and base of the smear.
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I hope you can see the smear well enough in the video if not please leave a comment and. The portion of blood film near the drop of blood. They then tie a band above. Take one drop of venous or capillary blood. Select the finger to puncture usually the middle or ring finger.
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The feathered edge the monolayer and the body and base of the smear. Common cause of a poor blood smear. In infants puncture the heel. Blood Smear Technique 1 On one slide the sample slide place a small drop of blood 2 Use the second slide as a spreader slide. The tapering end of the blood film.
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A phlebotomist a person specifically trained to draw blood first cleans and sterilizes the injection site with an antiseptic. A quick instructional video demonstrating. The main part of the blood film. Always start with room temperature well-mixed clot free EDTA anticoagulated blood heparin is used for some exotic species. Drop of blood too large or too small 2.
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Can occur with anemic patients Increase the angle of the slide you are using to smear with. The main part of the blood film. Do not push the blood by having it ahead of the smearing slide. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. Otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge and your smear will be so thin you will have trouble finding enough cells.
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I hope you can see the smear well enough in the video if not please leave a comment and. The main part of the blood film. I hope you can see the smear well enough in the video if not please leave a comment and. In this video I quickly go over how to make a peripheral blood smear. The process is similar to making a normal thick film but instead of using a stick to spread the blood the edge of a glass microscope slide is used while applying firm pressure to create small scratches in the underlying slide.
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